Identification of Breakpoints in t (8;21) Acute Myelogenous Leukemia and Isolation of a Fusion Transcript, AMLl
نویسنده
چکیده
We have developed a restriction map of the chromosome 21 breakpoint region involved in t(8;21)(q22;q22.3) acute myelogenous leukemia (AML) and have isolated a genomic junction clone containing chromosome 8 and 21 material. Using probes from these regions, rearrangements have been identified in each of nine cases of t(8;21) AML examined. In addition, we have isolated cDNA clones from a t(8;21) AML cDNA library that contain fused sequences from chromosome 8 and 21. The chromosome 8 component, referred to as €TO (for eight twenty-one), is encoded over a large genomic region, as suggested by the analysis of corresponding yeast
منابع مشابه
Detection of the AML1/ETO Fusion Transcript in the t(8;21) Masked Translocation in Acute Myelogenous Leukemia I
The fusion transcript AML1/ETO was detected in the bone marrow of two t(8;21)-negative acute myelogenous leukemia (AML) patients by means of reverse transcription-polymerase chain reaction. This fusion transcript is identical to the one transcribed from the t(8;21) translocation base, as deduced from (a) the size and restriction pattern of the amplified DNA fragment and (b) the DNA sequence ana...
متن کاملDetection of the AML1/ETO fusion transcript in the t(8;21) masked translocation in acute myelogenous leukemia.
The fusion transcript AML1/ETO was detected in the bone marrow of two t(8;21)-negative acute myelogenous leukemia (AML) patients by means of reverse transcription-polymerase chain reaction. This fusion transcript is identical to the one transcribed from the t(8;21) translocation base, as deduced from (a) the size and restriction pattern of the amplified DNA fragment and (b) the DNA sequence ana...
متن کاملGeographic Heterogeneity of the AML1-ETO Fusion Gene in Iranian Patients with Acute Myeloid Leukemia
Background: The human AML1 gene, located on chromosome 21, can be fused to the AML1- eight-twenty-one (ETO) oncoprotein on chromosome eight, resulting in a t(8;21)(q22;q22) translocation. Acute myeloid leukemia (AML) associated with this translocation is considered a distinct AML with a favorable prognosis. Due to the various incidences of the translocation, which is associated with geographic ...
متن کاملPersistence of the 8;21 translocation in patients with acute myeloid leukemia type M2 in long-term remission.
The translocation between chromosomes 8 and 21, t(8;21) (q22;q22), is the most frequent abnormality in acute myeloid leukemia (AML) with French-American-British type M2 (FAB-M2) morphology. The breakpoints in this translocation have been characterized at the molecular level, and the genes involved are AML1 on chromosome 21 and ETO on chromosome 8. The rearrangement of the two chromosomes result...
متن کاملRAPID COMMUNICATION Persistence of the 8;21 Translocation in Patients With Acute Myeloid Leukemia Type M2 in Long-Term Remission
The translocation between chromosomes 8 and 21, t(8;21) (q22;q22), is the most frequent abnormality in acute myeloid leukemia (AML) with French-American-British type M2 (FAB-MP) morphology. The breakpoints in this translocation have been characterized at the molecular level, and the genes involved areAML 1 on chromosome 21 and €TO on chromosome 8. The rearrangement of the two chromosomes result...
متن کامل